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. 2011 Aug 22;108(35):14694–14699. doi: 10.1073/pnas.1105685108

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Fig. 6. — Regulation of RPS2-mediated resistance and RPS2 protein accumulation by CPR1. (A and B) Growth of P. syringae pv. tomato DC3000 (A) and P. syringae pv. tomato DC3000 carrying avrRpt2 that is recognized by RPS2 (B) in two independent transgenic lines (T1-4 and T1-5) overexpressing CPR1 in wild-type Col background. WT, eds1-2, and ndr1-1 plants were used as controls. (C) Western blot analysis of RPS2-HA protein levels in two CPR1-FLAG transgenic lines (T1-7 and T1-18) expressing CPR1-FLAG under its own promoter in a previously described RPS2-HA transgenic background (20). The weak band in the RPS2-HA lane is the result of nonspecific hybridization from anti-FLAG antibody. (D) Western blot analysis of RSP2-HA protein levels in cpr1-3. The RPS2-HA transgene was crossed into cpr1-3. (E) Expression levels of the RPS2-HA transgene in RPS2-HA and cpr1-3 crossed into RPS2-HA.