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. 2011 Aug 22;108(35):14694–14699. doi: 10.1073/pnas.1105685108

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Fig. 7. — Interactions between CPR1 and SNC1 (A) or RPS2 (B). (A) In vivo pull-down of SNC1-HA by CPR1-FLAG. (B) In vivo pull-down of RPS2-HA by CPR1-FLAG. (C) Immunoprecipitation of SNC1-HA or snc1-HA and ubiquitination levels of SNC1-HA or snc1-HA proteins. Arabidopsis mesophyll protoplasts were transfected with the indicated constructs. Total protein extracts were subjected to immunoprecipitation with anti-FLAG agarose beads (A and B) or anti-HA microbeads (C). Crude lysates (Input) and immunoprecipitated proteins (Elute) were detected with anti-FLAG, anti-HA, or anti-Ub antibodies.