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. 2011 Aug 22;108(35):14566–14571. doi: 10.1073/pnas.1112317108

Fig. 5.

Fig. 5.

Tet2-deficient hematopoietic stem cells retain sustained progenitor properties in vitro but preferentially undergo monocyte/macrophage differentiation. (A) Effect of Tet2 deficiency on myeloid differentiation. LSK cells isolated from bone marrow of Tet2+/+ or Tet2−/− mice were grown in the presence of cytokine mixtures as shown. After 5 d, flow cytometric analysis was performed for the expression of lineage markers (Upper) and Gr-1 and Mac-1, myeloid markers (Lower). Data are representative of at least two independent experiments (n = 3–5). (B) The cells in A were analyzed for their expression of CD115 (M-CSFR) and F4/80, monocyte/macrophage markers (Upper). The CD115+F4/80+ cells were further assessed for their expression of myeloid markers (Lower). Data are representative of at least two independent experiments (n = 3–5).