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A positively charged patch in the effector domain, but not the RXLR domain, of Avr1b is required for PIP binding. (A) Positions of positively charged amino acids Lys75, Lys79, Lys80, and Lys83 in the Avr1b structure. (B) PIP binding of the RXLR mutants Avr1bRFLR/AAAA and Avr1bRFLR/QFLR and AVR1b with mutations at the positively charged patch, Avr1bK79E, and Avr1bK79E, K83E. The lipid overlay assay was performed as in Fig. 2A.
