Fig. 3.

Comparative catalysis at 25 °C of the wild-type and Q¹⁴⁹A-V²⁶⁵I mutated tobacco (L^t₈S^t₈) rubisco, the source Flaveria (L^F₈S^F₈) rubiscos, the hybrid (L^F₈S^t₈, comprising Flaveria L- and tobacco S-subunits), and chimeric (L^cF₈S^t₈, comprising chimeric Flaveria L- and tobacco S-subunits) rubisco variants produced in the transplastomic tobacco plants. The L-subunit amino acid residues at codons 149, 265, and 309 in each rubisco type are shown. Values shown are the average ± SD of independent assays (n = 4–24; see Materials and Methods for details). The maximal oxygenation rate (V_O) was calculated using the equation S_C/O = (V_C/K_C)/(V_O/K_O).