Figure 4.

Identification of RNA binding proteins. (A) Northwestern blot analysis performed with 3-DRA probe and 3-DRB1 of Raji cell extracts after Mono Q chromatography. The lanes indicated with numbers 37 and 56 represent the fractions showing the binding; the identified proteins are indicated. The molecular weight markers are shown in the middle. (B) SDS–PAGE analysis of heparin-sepharose pool b, indicated as H(b) and of fractions 37 and 56. The gel was stained with coomassie blue. (C) western blot analysis with anti-EBP1 and anti-DRBP76 antibodies, after stripping the membranes analysed by northwestern blot.