Table 1.
Sequences of primers used for riboprobes template synthesis, target sequences of siRNA and sequences of primers used for qRT–PCR, respectively
| Probe | Primers | Sequence |
|---|---|---|
| Primers used for PCR of riboprobes templates | ||
| 3-DRA | 3DRAT7 | TAATACGACTCACTATAGGACATGGAGGTGATGGTGTTTC |
| 3DRAR | TTCCACCCAAGATCATCAAA | |
| 3-DQA1 | 3DQA1T7 | TAATACGACTCACTATAGGCCATCCTGGAAGGGAAGTG |
| 3DQA1R | TCAGGAGGTCAGGGAAAGAA | |
| 3-DRB1 | 3DRB1T7 | TAATACGACTCACTATAGGAATGCAGATGACCACATTCAAG |
| 3DRB1R | TGAGAAACATTTAATAATGTAATG | |
| 3-DQB1 | 3DQB1T7 | TAATACGACTCACTATAGGGCACTGACTCCTGAGACTATT |
| 3DQB1R | TGCTTCTCTTGAGCAGTCTGAG | |
| GFP | GFPT7 | TAATACGACTCACTATAGGAAGAGTGCCATGCCCGAAGGT |
| GFPR | GCTGCTGGGATTACACATGGC | |
| siRNA target sequences | ||
| siNF90 | 5′-GCCCACCTTTGCTTTTTAT-3′ | |
| siEBP1 | 5′-TCCCACCAGCATTTCGGTAAA-3′ | |
| siDRA | 5′-CAGGAATCATGGGCTATCAAA-3′ | |
| siDRB1 | 5′-CCTGGACAGATACTTCTATAA-3′ | |
| Primers used for qRT–PCR | ||
| β-Actin | ACT-F | TCATGAAGTGTGACGTTGACA |
| ACT-R | CCTAGAAGCATTTGCGGTGCAC | |
| GAPDH | G-F | AACGGATTTGGTCGTATTGGGC |
| G-R | TCGCTCCTGGAAGATGGTGATG | |
| HLA-DRA | DRA-F | GGACAAAGCCAACCTGGAAA |
| DRA-R | AGGACGTTGGGCTCTCTCAG | |
| HLA-DRA 3′UTR | 3DRA-F | ACATGGAGGTGATGGTGTTT |
| 3DRA-R | AGCCAGCTAGATGTTAGAGTACG | |
| HLA-DRB1 | DRB1-F | CTCAGCATCTTGCTCTTGTGCAG |
| DRB1-R | CAGCATTAAAGTCAGGTGGTTCC | |
| HLA-DQA1 | DQA1-F | GGTGTAAACTTGTACCAGT |
| DQA1-R | GGAGACTTGGAAAACACT | |
| HLA-A-B-C | MHCI_F | CTCAGCATCTTGCTCTTGTGCAG |
| MHCI_R | ATGTAATCCTTGCCGTCGTA | |
| EBP1 | EBP1-F | CTGCACGCCAATAGAAGG |
| EBP1-R | AGTAAACGGCATGGCATCA | |
| DRBP76/NF90 | NF90-F | CTGGTGCTGCTGTGTAAGGA |
| NF90-R | AGGGACAATGGAGGCTCTTT | |
The underlined sequence represents the T7 promoter.