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. 2011 May 20;39(16):7194–7208. doi: 10.1093/nar/gkr152

Figure 4.

Figure 4.

Importance of the length and sequence of the tail domain. (A) Western blots showing SMN and tubulin proteins after transfection of SMA fibroblasts with oligonucleotides containing different lengths of tail, as labelled above the lanes (oligonucleotide 1, 6 GGA motifs; oligonucleotide 3, 5 GGA motifs; oligonucleotide 6, 3 GGA motifs). The figures below the lanes show the normalized ratios of SMN:tubulin. (B) Qualitative PCR of the spliced isoforms of endogenous SMN2 mRNA after transfection of SMA fibroblasts by the designated oligonucleotides. The site annealed refers to the 5′-most nucleotide in the exon to which the oligonucleotide is complementary. (C) Qualitative PCR illustrating the effectiveness of different tail sequences.