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. 2011 Jun 7;39(16):e106. doi: 10.1093/nar/gkr442

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Cre-lox dependent DRCR amplification in yeast. (A) Structure of the m2s–loxPs cassette and a model for DRCR amplification. The sizes (kb) of the three regions in the structure are indicated below. A possible model for termination of the DRCR process is provided in Supplementary Figure S2D. (B) Frequency of Leu⁺ colony formation. CRE: Cre-induced condition; Δcre: negative control. (C) Southern analysis of a representative sample of m2s–loxPs survivors with the leu2d probe. The lanes marked in red and green indicate HSR- and DM-type products, respectively. Supplementary information, including the description of the lanes marked in gray, is provided in Supplementary Texts. M: S. cerevisiae marker; P: the parental strain (LS20); NS: non-selective conditions. (D) Southern analysis of SmaI-digested DNA of the samples from (C) with the leu2d probe. (E) Southern analysis of a representative sample with DM-type products by PFGE for lower range. (F) The predicted structure of the 18, 29 and 40 kb DM-type products. The SmaI sites (vertical lines) and the sizes (kb) of fragments that hybridize with the leu2d probe are shown. The gray arrows below indicate an inverted structure. (G) Schematic representation of the expected structure derived through the DRCR process and SmaI-restriction maps of the representative HSR-type structure. Inverted repeats that can engage in DRCR-dependent inversion are marked with red backgrounds. The gray arrows indicate a palindromic structure.