Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jun 7;39(16):e106. doi: 10.1093/nar/gkr442

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Gene amplification in CHO cells induced by Cre recombination. See details in the text and Supplementary Materials and Methods. (A) Structure of the modified BAC and construction of the CHO strain for gene amplification. The sizes (kb) of the three regions in the structure are indicated below. (B–K) Metaphase FISH analysis with FITC-labeled probes (green). The CHO DR1000L-4N (CHO-4N) strain (B) that contains approximately 170 copies of DHFR was probed with a pSV2-dhfr plasmid. The BAC-CHO strain (C; 1B12) without Cre induction and MTX selection and MTX-resistant clones (D–K) were probed with the BAC in (A). DNA is counterstained with DAPI (blue). The scale bars represent 10 µm. These amplified products would be derived from the integrated BAC construct because the BAC probe could not detect the original DHFR locus (Supplementary Texts). (L) A model for the HSR amplification and DMs production by Cre recombination coupled with replication. See details in the text.