Skip to main content
. 2011 May 18;39(16):7114–7123. doi: 10.1093/nar/gkr259

Figure 2.

Figure 2.

Gel shift studies of single stranded DNA oligonucleotides bound with PNAs. 5′-32P labeled BCL2single-C was incubated with cPNA1 (A), cPNA2 (B) and cPNA3 (C) in TE buffer with 20 mM KCl (pH 7.4) at 37°C overnight at varying molar ratios; numbers on the top indicate PNA concentration; DNA concentration was 5 nM. Samples were run in 20% native polyacrylamide TBE gels at room temperature. (D) Binding specificity studies. 5′-32P labeled BCL2single-C and BCL2single-C-m were incubated with cPNA1 (2, 6), cPNA2 (3, 7) and cPNA3 (4, 8) in TE buffer with 20 mM KCl (pH 7.4) at 37°C overnight at 30 nM PNA and 5 nM DNA concentration; BCL2single-C without PNAs was used as a control (1).