Figure 6.

PNA invasion studies. Chemical probing of plasmid with BCL2 insert with PNAs (A—cPNA1; B—cPNA3). Incubation of plasmids with PNAs was performed in TE plus 20 mM KCl (pH 7.4) at 37°C overnight with molar ratio 100:1 PNA to DNA. Experimental samples were incubated with central PNAs only and with both central PNA and bis-PNA. Plasmids with two BCL2 insert were tested: with original quadruplex forming BCL2 sequence and with mutant BCL2 sequence not forming quadruplex. G-rich strand was analyzed.