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. 2011 May 26;39(16):7020–7033. doi: 10.1093/nar/gkr157

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — ColE1 plasmids used for library generation. Diagrams present the ColE1 plasmids used to generate the pol I libraries. Shown are their main features: plasmid ori, ssi primosome assembly site signals, and ORFs (boxes). Sequence coverage (double-headed arrows), and directionality of replication and of sequencing (solid arrow) are also shown. The distance from the RNA/DNA switch is indicated, at 500 bp intervals. (a) hTK libraries: human thymidine kinase gene cloned in the TOPO vector, (b) pGFPuv libraries, bearing the cycle 3 GFP gene.