Table 1.

Pol I mutation libraries used in this study

Library	No. of rounds of mutagenesis	No. of clones sequenced	No. of bases sequenced (kb)	Total mutations	Total curated mutations
Human thymidine kinase
TK1	5-7	88	91	396	280
	5	23	24	81	65
	6	22	23	119	88
	7	43	44	196	127
TK2	7	65	43	148	113
Green fluorescent protein
GFP 1	1	276	305	184	184
		Day 1: 91	Day 1: 98	Day 1: 36
		Day 3: 185	Day 3: 209	Day 3: 148
GFP 2	2	63	64	60	60