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. 2011 Jun 17;39(16):e108. doi: 10.1093/nar/gkr465

Figure 3.

Figure 3.

3′ and 5′ BDs of selected RTMs. The BD sequences have a great influence on the efficiency of trans-splicing. Five RTMs selected from an RTM library with the same 3′ BD for intron 51 (a; binding position from nt 106 of intron 51 to nt 257 of intron 51) of COL17A1 but with different BDs for the exon 52—intron 52 target region were selected for further testing. RTM 0 had no 5′-BD, whereas RTM 2 and 4 harbored two separate BDs specific for exon 52—intron 52. One region of complementarity of RTM 2 masks the exon/intron boundary of exon 52—intron 52 (c; binding position from nt 360 of exon 52 to nt 190 of intron 52). The second domain repeats some of same region of the 5′-end of intron 52 (d; binding position from nt 20 to nt 63 of intron 52). RTM 4 binds to the central (f; binding position from nt 318 to nt 338 of intron 52) and 3′ (g; binding position from nt 457 to nt 555 of intron 52) portion of intron 52. RTMs 1 and 3 each have one 5′ BD complementary to the central portion of intron 52 (b; nt 217 to nt 297 of intron 52, e; nt 191 to nt 297 of intron 52).