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. 2011 May 17;39(16):6908–6918. doi: 10.1093/nar/gkr303

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — MARs are active chromatin regions. (A) Chromatin from IMR-32 and MEFs were extracted and immunoprecipitated with acetylated histone 3 lysine 9/14. The immunoprecipitates were then analyzed for amplification of miR-17–92 cluster and the upstream and downstream MAR elements. Rabbit IgG served as negative control. Equal amounts of starting material were used, as verified using 10% total lysate as input. (B) Chromatin immunoprecipitates from IMR-32 and MEF cells were analyzed for histone 3 lys 9 trimethylation (H3K9 me3) or acetylation (H3K9/14 Ac) of the upstream and downstream MARs (US1 and DS) and control element (US2) to verify the epigenetic status of these regions. (C) Chromatin immunoprecipitate from IMR-32 cells were analyzed for the histone acetylation status of the MAR elements for the indicated miRNAs. A quantity of 10% of total lysate served as input. Pre-immune sera served as negative control.