Figure 1.
Characterization of the lic gene. Complementation of a pbs2 deletion mutant in Saccharomyces cerevisiae using lic cDNA. (A) Yeast strain TM334 (pbs2) was transformed with various plasmids as indicated. Transformants were streaked onto YPD plates containing 1 m sorbitol and incubated at 30°C. (B) Yeast strain TM334 (pbs2) was transformed with empty vector (−) or an expression vector for HA–LIC (+). Cells were treated with NaCl (0.5 m) for the indicated times. Immunoprecipitated complexes obtained with anti-HA were used for in vitro kinase reactions with GST–p38-KN as a substrate (top). The amounts of immunoprecipitated HA–LIC were determined with anti-HA (bottom). (C) Complete sequence of full-length lic cDNA (accession no. AJ238572) and a phylogenetic tree showing the relationship between lic and other Drosophila and human MAPKKs. lic is most homologous to the human MKK3 and MKK6 p38 activators (D).