Figure 2.

Molecular organization and expression of lic. (A) Genomic organization of the {hep, lic} locus. hep1 is a mobile P element inserted in the hep 5′ untranslated region, and was used to generate double {hep, lic} mutant alleles by imprecise excisions. The lines above the restriction map indicate the DNA lesions associated with five {hep, lic} alleles, as determined by Southern blot analysis. The solid boxes indicate DNA deletions within the two adjacent EcoRI fragments covering hep and lic. Below the restriction map is represented a schematic structure of full-length lic cDNA. Broken lines indicate the introns; solid boxes represent the coding region. (E) EcoRI. lic is expressed throughout development at variable levels. Expression in early (0–2 hr) embryos indicates a maternal contribution. The same blot was hybridized using a ribosomal gene probe (rpL17A) to assess mRNA levels. (C) In situ hybridization to wild-type ovaries indicates germ-line expression in nurse cells from stage 7–8 onward. (D) In {hep, lic}H6 germ-line clones, lic mRNAs are not detected. lic expression in the germ line can be restored using a UBlic transgene as shown in E.