Figure 5.

lic is required for the maintenance of osk mRNA posterior localization in the oocyte. In situ hybridization of wild-type (A,B) and lic mutant (C–E) oocytes at stages 9 (A,C,D) and 12 (B,D) of oogenesis. (A,B) In wild type, osk mRNA is localized posteriorly in the oocyte until the end of oogenesis. (C) In lic mutant egg chambers, osk mRNA normally localizes at the posterior pole of the oocyte but is not properly maintained and diffuse toward more anterior regions. (Arrowhead in D) In some cases, osk mRNA also accumulates centrally. (Arrowhead in E) osk transcripts are barely detectable in stage 12 oocytes, indicating that diffusion of osk proceeds continuously throughout oogenesis. Anterior is on the left.