Figure 6.

lic controls grk activity in mid-oogenesis. Immunostaining of wild-type (A) and lic (D,G) stage 9 oocytes using an anti-grk antibody (lateral views; dorsal is up). (D) In mutant oocytes, grk protein is mislocalized. The egg chamber in G represents the most extreme localization defect that we have observed. β-Galactosidase staining of wild type (B) and lic (E,H) stage 10 egg chambers shows kek expression in the dorsal region of the follicle cells (45%; n = 49). Note that in lic mutants, kek expression is affected, both in terms of levels and shape of the expression domain. In rare cases, kek expression is expanded laterally and ventrally, suggesting a dorsalization of the future egg chorion (I; <5%). (C,F,I) The chorion phenotypes corresponding to grk and kek expression patterns in wild-type (C) and lic egg chambers (F,I; 50% and <1%, respectively; n = 219). B, C, E, F, and I are dorsal views. H is a lateral view, with dorsal up. For all panels, anterior is on the left.