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. 1998 May 1;12(9):1338–1347. doi: 10.1101/gad.12.9.1338

Figure 1.

Figure 1

 Proteins used for degradation studies of SsrA-tagged proteins. Cartoon representation of the structures of hybrid proteins. The names of these proteins and of the plasmids that encode them are indicated to the left. [λ(1-93)] The 93-residue sequence of the amino-terminal, operator-binding domain of the λ cI repressor. (M2 Flag) The epitope sequence DYKDDDDK. (6His) The sequence HHHHHH. (SsrA tag AA) The sequence AANDENYALAA; (SsrA tag DD) The sequence AANDENYALDD. (trpAt) The AARLMSG sequence encoded by the stem–loop region of the trpAt transcriptional terminator. Note that because of heterogeneity in the position of SsrA tagging (Keiler et al. 1996), the λN–trpAt protein may consist of several species, some of which differ by 1 or 2 amino acids from the otherwise identical sequence of λN-L2-AA.