Figure 4.

DNase I sensitivity at the β-globin LCR HSs, γ-globin promoter and 3′HS1 in the GATA-1 or p45/NF-E2 knockdown cells. (A) Nuclei of K562 cells expressing each shRNA were digested with 150–600 U DNase I. DNA extracted from the digest was run on 1% agarose gel. (B) DNase I sensitivity was determined by quantitatively comparing digested DNA with undigested DNA and normalizing to the sensitivity at the Actin gene at each concentration. The results are averages of two to four nuclei preparations ± SEM. (C) ChIP was performed using Brg1 antibody described in Figure 1.