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. 1999 Jun 1;13(11):1367–1381. doi: 10.1101/gad.13.11.1367

Figure 5.

Figure 5

Figure 5

A dominant-negative mutant of FADD confers increased resistance to microinjected cytochrome c and inhibits c-Myc-induced caspase activation. (A) (●) Rat-1/c-MycER and (○) Rat-1/c-MycER/FADD DN fibroblasts grown in 10% FCS were microinjected with 25 μm pure hcC mixed with dextran-coupled rhodamine dye and the number of injected cells then evaluated by fluorescence microscopy. Cells were incubated at 37°C and, at the indicated time points, the number of nonapoptotic cells was evaluated and expressed as percentage of the initial number of injected cells. Results presented are the mean ±s.e.m. of five independent experiments, each of ∼100 injected cells. (B) Rat-1/c-MycER and Rat-1/c-MycER/FADD DN fibroblasts were serum-deprived prior to addition of OHT (100 nm). Where indicated, IGF-1 (100 ng/ml) was added simultaneously. Twenty-four hours later, postmitochondrial fractions were prepared and assayed for DEVD cleaving activity as described in Materials and Methods. Results presented are the mean ±s.e.m. of at least three independent experiments.