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Degradation of cI105 and cI–SsrA by both HflB and Clp. The strains used were JM105 (WT) (○), its hflB1 derivative (•), its clpP::Tn9 derivative (▵), and its hflB1 clpP::Tn9 derivative (▴). (A) These strains carry plasmid p105 (cI105); (B) they carry plasmid pλN-SPT (cI–SsrA); a wild-type control (+) carrying plasmid pDP102 (cI102), coding for a stable cI variant, is shown in A and B. Cultures were grown, pulse labeled, chased, and analyzed as in the experiments shown in Fig. 1.
