Figure 2.

Yeast two-hybrid interaction of FEM-3 and the carboxy-terminal region of TRA-2A. (A) Diagram of fragments of FEM-3 and TRA-2A used in this study. Numbers indicate the amino acids at the termini of each fragment. The predicted signal sequence and transmembrane domains of TRA-2A are indicated as filled rectangles. The MX region is a 22-amino-acid region defined by tra-2 mutations that transform XX hermaphrodites into females but do not affect XO animals. (Kuwabara et al. 1998). (B) Interaction between FEM-3 and TRA-2A fragments in the yeast two-hybrid system. β-Galactosidase activity was measured in liquid cultures of at least four independent transformants. Activity was normalized to the level in cells coexpressing a FEM-3 DNA-binding domain fusion and a hybrid of the Gal4 activation domain and Snf4. Snf4 and Snf1 are yeast proteins that served as negative controls for interactions involving FEM-3 and TRA-2A.