Figure 3.
Coimmunoprecipitation of the carboxy-terminal region of TRA-2A with MycFEM-3. [35S]Methionine-labeled MycFEM-3 (lanes 1,2), TRA-2AΔ1 (lanes 3,4) or both MycFEM-3 and TRA-2AΔ1 (lanes 5,6) were produced by in vitro translation in reticulocyte lysates. Equivalent samples were analyzed directly by SDS-PAGE and fluorography [(T) lanes 1,3,5] or subjected to immunoprecipitation with anti-Myc mAb 9E10 prior to electrophoresis [(IP) lanes 2,4,6]. The solid arrowhead marks the position of MycFEM-3; the open arrowhead marks the position of TRA-2AΔ1. Numbers at right indicate the positions and the relative molecular mass in kilodaltons of marker proteins.