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Masculinization of fem-3 XO and XX mutants by HS–MycFEM-3. Animals of genotypes unc-24 fem-3; idIs4/O XO and unc-24 fem-3; idIs4/+ XX (solid bars), or unc-24 fem-3; idIs4/+ XX only (hatched bars) were subjected to repeated heat shocks during development as described in Materials and Methods. No sign of male development was observed in the absence of heat shock treatment.
