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. 1999 Jun 1;13(11):1453–1463. doi: 10.1101/gad.13.11.1453

Figure 6.

Figure 6

Expression of MycFEM-3 and truncated derivatives in transgenic nematodes. Lysates were prepared from animals maintained continuously at 20°C (no heat shock; HS −, lanes 2,4,6) and from animals subjected to heat shock at 33°C followed by a 2-hr recovery period at 20°C (HS +, lanes 1,3,5,7). The lysates were analyzed by SDS-PAGE and immunoblotting with antibody 9E10. Transgenic nematodes carried plasmids encoding MycFEM-3 (FL, lanes 1,2), MycFEM-3Δ1 (Δ1, lanes 3,4), or MycFEM-3Δ2 (Δ2, lanes 5,6), or empty heat shock vector (lane 7). Numbers at right indicate the relative molecular masses in kilodaltons and the positions of migration of molecular mass markers.