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. 1999 Jun 1;13(11):1453–1463. doi: 10.1101/gad.13.11.1453

Figure 7.

Figure 7

Suppression of the masculinizing effect of HS–MycFEM-3 by the carboxy-terminal domain of TRA-2A. (A) Masculinization of the gonad in XX animals expressing HS–MycFEM-3 with an inactive TRA-2A construct, HS–TRA-2Afs (hatched bars; three transgenic lines, n = 195) or with HS–TRA-2AΔ1 (stippled bars; five transgenic lines, n = 414). (B) Expression of MycFEM-3 in the transgenic lines used in A. Lysates from lines coexpressing MycFEM-3 and TRA-2AΔ1 (lanes 1–5) or from lines expressing MycFEM-3 and TRA-2Afs (lanes 6–8) were analyzed by SDS-PAGE and immunoblotting with the anti-Myc mAb 9E10 to test whether they contained similar levels of MycFEM-3. An equivalent blot was probed with a FEM-2 antiserum to verify that similar quantities of protein were present in each sample.