Table 1.
Masculinization of XX animals by a TRA-2Abinding fragment of FEM-3
|
HS–MycFEM-3 varianta
|
No.
|
Percent masculinization of
|
||
|---|---|---|---|---|
|
gonad
|
ventral hypodermis
|
tail
|
||
| Nonebc | 45 | 0 | 0 | 0 |
| FEM-3 | 50 | 100 | 94 | 88 |
| FEM-3Δ1 | 167 | 42 | 23 | 7 |
| FEM-3Δ2c | 194 | 0 | 0 | 0 |
a
Each transgenic line carried the indicated heat inducible transgene on an extrachromosomal array. Number of independent lines analyzed: vector, 4 lines; FEM-3, 3 lines; FEM-3Δ1, 10 lines; FEM-3Δ2, 9 lines.
b
The extrachromosomal array carried the heat shock promoter plasmid pPD49.83 with no insert.
c
One animal had abnormal gonadal morphology, but it was not recognizably masculinized.