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Masculinization by HS–MycFEM-3 requires fem-1 activity
| Cross: dpy-13/fem-1 unc-24; idIs4 XO × fem-1 unc-24; idIs4 XXa | |||||
|---|---|---|---|---|---|
| Non-Unc F1 | Unc F1 | ||||
| male | intersex | female | male | intersex | female |
| 214 | 171 | 12 | 0 | 3 | 350 |
unc-24 maps ∼1.5 map units from fem-1. Hence, ∼99% of the Unc F1 progeny were homozygous for fem-1.
