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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Am Acad Dermatol. 2011 Apr 29;65(1):25–34. doi: 10.1016/j.jaad.2010.09.016

Fig 1.

Fig 1

Identification of patients with anti-melanoma differentiation-associated gene 5 (MDA5) antibodies. Plasma samples from patients with dermatomyositis (DM) and healthy control subjects were used to immunoprecipitate radiolabeled MDA5 generated by in vitro transcription/translation. All 10 of anti-MDA5 patient samples are shown (lanes 1–10), as are 3 DM samples that are anti-MDA5-negative (lanes 11–13) and 3 samples from healthy individuals (lanes 14–16). Leftmost lane (Input) refers to MDA5 protein loaded with no immunoprecipitation. Molecular weight markers (left).