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. 1998 May 1;12(9):1248–1253. doi: 10.1101/gad.12.9.1248

Figure 3.

Figure 3

 RadA protein can promote the formation of joint molecules. (A) DNA substrates and the expected product of the reaction. (B) An agarose gel displaying a time course for formation of joint molecules. The first six lanes show RadA protein-dependent D-loop formation with the 54-mer oligonucleotide SKBT17 at 65°C; the second six lanes show RadA protein-dependent D-loop formation with the 54-mer SKBT16 at 65°C; the third six lanes show the same reaction with SKBT16 in the absence of protein at 65°C; the last two lanes show RecA protein-dependent D-loop formation with SKBT16 at 37°C. (C) Joint molecule formation is dependent on RadA protein and DNA sequence homology. The error bars indicate the s.d. for three sets of experiments. (▪) SKBT16; (▵) SKBT17; (▾) PBR322 heterologous control; (⋄) no RadA.