Figure 3.

Percentages of CD8+ (A and B) and CD4+ cells (C and D) in murine lymph node cell cultures after incubation with synthetic peptides derived from the COOH-terminal extension of L. (L.) amazonensis CPB. Cell cultures from 15 infected BALB/c (A and C) or CBA (B and D) mice were incubated for 3 days in 24-well plates with synthetic peptides (30 μg/mL), PLA (30 μg/mL) or Con A (2 μg/mL), stained with specific CD4 and CD8 antibodies conjugated to fluorophores and submitted to analysis by flow cytometry. Results are from a representative assay from at least three independent repeats. *Statistically distinct from control (p < 0.05).