Table 4.
Predicted interactions in MHC/peptide complexes related to cytokines production in murine lymp hnode cell cultures
| IL-10 | IL-12 | IFN-γ | IL-10 + IL-12 | IL-10 + IFN-γ | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Specific MHC | Interactions | HB | VWC | HB | VWC | HB | VWC | HB | VWC | HB | VWC |
| H-2Kd | Exclusive | tyr 7 | cys 164 | *NP | *NP | *NP | phe 33 | *NP | *NP | tyr 171 | try 133 gly 149 |
| Predominant | try 73 tyr 159 |
*NP | try 147 | *NP | gly 63 | *NP | *NP | *NP | tyr 156 | *NP | |
| H-2Kk | Exclusive | arg 155 H2O 373 | *NP | *NP | gly 65 gly 69 phe 74 | *NP | *NP | tyr 45 | asp 152 | *NP | *NP |
| Predominant | *NP | *NP | asp 156 | asn 70 | *NP | *NP | *NP | *NP | *NP | *NP | |
Hydrogen bonds (HB) and Van der Waals contacts (VWC) were predicted by in silico Molecular Docking assays. Exclusive interactions (ExI) were predicted only between the specified murine strain H-2K molecule and the peptides that induce the discriminated cytokine production in lymph node cell cultures. Predominant interactions, despite not being exclusive, were predicted to occur at least twice more frequently for specific cytokine production-related peptides. *NP - none predicted.