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. 2011 Sep 6;5(9):e1313. doi: 10.1371/journal.pntd.0001313

Figure 5. IFN-γ production by splenocytes of immunized mice.

Figure 5

BALB/c mice were orally immunized 3 times, 2 weeks interval, with nirB strain, pagC strain, pMohly strain, vector strain or PBS (control). The splenocytes of immunized mice were collected at 2 weeks after the 3rd immunization and stimulated with recombinant Sj23LHD protein (10 µg/ml), GST protein (10 µg/ml), conA (10 µg/ml) or media alone. The content of IFN-γ in the culture supernatant was measured by ELISA kit. Each column represents the mean±SD of 3 independent experiments. ***P<0.001 when compared with vector group.