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. 2011 Sep 6;6(9):e22842. doi: 10.1371/journal.pone.0022842

Figure 2. TGF-β antagonises IL-15 induced gene expression of NK cell activation receptors.

Figure 2

(A) Cell surface expression of NK cell activation receptors (as indicated) by unstimulated NK cells (green histogram), IL-15 stimulated NK cells (red histogram) or NK cells treated with IL-15 and TGF-β (blue histogram). Cells were cultured for 48 hrs. Isotype control stains are shown in purple. (B) Receptor gene expression in cultures of unstimulated NK cells (U), IL-15 stimulated cells (15) and IL-15 plus TGF-β treated NK cells (15+β). Steady-state mRNA levels were determined by quantitative (q)RT-PCR and the results expressed as arbitrary units (with expression in unstimulated NK cells defined as 1 unit; note the different scales). The colours correspond to the flow cytometry plots shown in (A).