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. 1998 May 1;12(9):1315–1326. doi: 10.1101/gad.12.9.1315

Figure 8.

Figure 8

 Role of Ets2 in FGF2 induction of MMP-13 and MMP-3 in cultured cells. The EKO1 Ets2-deficient fibroblastic cell line and two independent isolates that expressed transfected Ets2 (E8 and E13) were incubated in medium containing 0.5% fetal bovine serum for 2 days. FGF2 (50 ng/ml) was added for 7 hr and RNA was isolated. The indicated RNAs were detected by RNase protection analysis. Collagen IV is indicated by Col4. Shown are autoradiographic exposures of the acrylamide gels used to separate the protected fragments. The time of growth factor exposure is indicated in hours at the top of each lane. Note the increased expression of the MMP-3 (A, lanes 3,5) ) and MMP-13 (B, lanes 3,5) RNAs in the E8 and E13 cell lines and their further increase after 7 hr.