Figure 5. Endothelial commitment is increased in ESC knockdown lines.

A) ESC lines stably expressing shRNA sequences specific to E-cadherin, Cx-43, ZO-1 and ZO-2 were differentiated in Collagen I with 100 ng/ml bFGF and 50 ng/ml VEGF for 11 days. B) Percentage of sprouting EB and C) number of sprouts per sprouting EB were manually measured on photomicrographs in ESC reduced in expression of junction molecules compared to D3-ESC control. *** p-value<0.0005, ** p-value<0.005; * p-value<0.05.