Figure 3. Distribution of GFP-CD63 containing exosomes in adherent and non-adherent BT-CD63 cells.

A-B) BT-CD63 cells were maintained in adherent (panel A) or non-adherent (panel B) modes as described in materials and methods and examined by confocal microscopy Note that the EDTA-induced detachment of BT-CD63 led to the accumulation of the GFP-CD63 labeled exosomes on the cell surface. C-D), parental BT-549 cells in adherent cultures or in anchorage-independent cultures on polyHEMA coated plates were incubated with conditioned medium containing GFP-CD63 vesicles for 3 h. Cells were thereafter processed for microcopy as described in Materials and Methods. Bar is 10 µm.