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. 2011 Sep 6;6(9):e23833. doi: 10.1371/journal.pone.0023833

Figure 3. D492M has acquired an EMT phenotype.

Figure 3

A, Immunofluoresence staining on D492M show switch from E- to N-Cad and reduced expression of K14 and K19. Counterstain TO-PRO-3, Bar 100 µm. B, Western blotting confirms downregulation of epithelial markers such as E-Cad, K-5/6, 8, 14, 17 and 19 in D492M. In contrast, the mesenchymal markers N-Cad, Vimentin and alpha-smooth muscle actin were expressed more intensively in D492M than D492. GAPDH loading control. C, EMT associated transcription factors are upregulated in D492M. Gene expression data showed upregulation of FOXC2 (3.96 fold,p: 8.4e-17) and FOXC1 (1.29 fold,p: 0.004) transcription factors in D492M. D, FOXC2 is strongly expressed in breast epithelial cell lines with EMT phenotype. Western blotting shows strong expression of FOXC2 in D492M and MDA-MB-231, an EMT-like breast cancer cell line, compared to no or low expression in D492 and D382. Actin, loading control.