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. 2011 Sep 6;6(9):e24283. doi: 10.1371/journal.pone.0024283

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Murrell et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic of Experimental Setup. Three inlets supply cell media to an epithelial sheet cultured in the wide, high channels. This is done under no flow. (B) After cells have reached confluency, 15 l/min flow () is applied, leading to three separated laminar streams. One stream contains 0.05% trypsin, while the other two contain cell media. This cleaves cells from the channel as shown in (C). On average, cleavage takes 5 min. Images are acquired in brightfield.

Inline graphic — (A) Schematic of Experimental Setup. Three inlets supply cell media to an epithelial sheet cultured in the wide, high channels. This is done under no flow. (B) After cells have reached confluency, 15 l/min flow () is applied, leading to three separated laminar streams. One stream contains 0.05% trypsin, while the other two contain cell media. This cleaves cells from the channel as shown in (C). On average, cleavage takes 5 min. Images are acquired in brightfield.