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. 2011 Sep 6;6(9):e24283. doi: 10.1371/journal.pone.0024283

Figure 6. Velocity and Persistence are Hindered in the Submarginal Region of Enzymatically Denuded Sheets.

Figure 6

Displacement Vectors (by cell tracking) for sheets after enzymatic (A) or scratched (B) denudations (red arrows). L.E.: leading edge, S.M.: submarginal cells. The leading edge is hand drawn in gray. Grid size is 20 Inline graphicm. Straightness (C) and Speed (D) of cell movement as a function of distance from the (initial) leading edge, Inline graphic (N = 6). (E) Immunostaining of E-cadherin at T = 0, 4, and 12 hours post cleavage (N = 3). Scale bar is 50Inline graphicm. (F) Fluorescence intensity as a function of Inline graphic. Straightness (G) and Speed (H) as a function of Inline graphic for sheets blocked with 200Inline graphicg of anti-E-cadherin antibody (N = 2).