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. 2011 Sep 6;6(9):e24476. doi: 10.1371/journal.pone.0024476

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Johnson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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WT, three wild type lines of rice; OE-OsNAS1, 30 independent transgenic lines overexpressing OsNAS1; OE-OsNAS2, 39 independent transgenic lines overexpressing OsNAS2; OE-OsNAS3, 24 independent transgenic lines overexpressing OsNAS3. Unpolished grain was analyzed by ICP-OES to determine Fe and Zn concentrations. The three populations of overexpression lines are sorted in order from lowest to highest Fe concentration in panels A and B.