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. 2011 Sep 6;6(9):e24649. doi: 10.1371/journal.pone.0024649

Figure 3. Transcriptional activation of FaASR in yeast.

Figure 3

(A) Diagram showing all the reporter constructs for analysis of pGBKT7 (DNA-binding protein)-dependent transactivation of FaASR in the yeast strain AH109. The vectors were constructed with pGBKT7 (negative control), pGBKT7-53 + pGADT7-T (positive control), pGBKT7-FaASR-F (amino acids 1-192), pGBKT7-FaASR-N (amino acids 1–100), and pGBKT7-FaASR-C (amino acids 101–192). (B) Clones transformed with the different vectors were grown on SD plates with or without histidine and adenine for 3 d at 30°C. Transcription activation was monitored by the detection of yeast growth without histidine and adenine and β-galactosidase assay.