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L69,77A NS1 protein has altered subcellular localization compared with WT NS1 protein during viral infection. Vero cells were mock infected or infected with WT NS1 or L69,77A NS1 viruses at an m.o.i. of 5. Cells were fixed, permeablized at 9 and 12 h after infection. NS1 protein was detected with NS1 antibody in combination with an Alexa Fluor 568 conjugated secondary antibody. Bar, 10 µm.
