Fig. 2.

Akt kinase activity in KB (a, b) and OBA-9 (c, d) cells following EGF, T. forsythia or BspA treatment. (a, c) Cell extracts were analysed by Western blotting. Anti-Akt and anti-phospho-Akt antibodies were used as primary antibodies. (b, d) Densitometric analyses of immunoblots in (a) and (c) showing expression levels of phospho-Akt after various treatments relative to those of non-treated cells. Total Akt levels were used for normalization. Data were analysed with NIH ImageJ software. The tables below the bar graphs show fold changes observed for representative blots and two other independent replicates.