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. 2011 Aug;157(Pt 8):2382–2391. doi: 10.1099/mic.0.048975-0

Fig. 5.

Fig. 5.

T. forsythia invasion assay with (a) MβCD- and (b) nystatin-treated KB and OBA-9 cells. The number of bacteria that invaded non-treated KB cells was designated 100 %. Results are means and sems of three independent experiments each with triplicate measurements. Asterisks indicate statistically significant differences relative to untreated cells (*, P<0.05; **, P<0.01; ***, P<0.001). (c) Relative expression of Cav-1 was assessed by qRT-PCR and Western immunoblotting. For qRT-PCR, the values were normalized with respect to β-actin expression. The Cav-1 expression of non-treated KB cells was designated 100 %. (d) T. forsythia and P. gingivalis invasion efficiency in Cav-1 knockdown KB cells. The number of bacteria that invaded non-treated KB cells was designated 100 %. Results are means and sems of three independent experiments each with triplicate measurements. *, P<0.05 relative to untreated cells.