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. 2011 Jul;157(Pt 7):2094–2105. doi: 10.1099/mic.0.046862-0

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© 2011 SGM

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — B. fragilis and B. thetaiotaomicron activate the contact system. (a) Binding of FXI to the surface of Bacteroides. Lanes: 1, native FXI; 2, B. thetaiotaomicron+FXI; 3, B. thetaiotaomicron+PBS; 4, B. fragilis+FXI; 5, B. fragilis+PBS; 6, prestained protein marker. The arrowheads point to activated conformations of FXI. (b) Binding of FXII to the surface of Bacteroides. Lanes: 1, native FXII; 2, B. thetaiotaomicron+FXII; 3, B. thetaiotaomicron+PBS; 4, B. fragilis+FXII; 5, B. fragilis+PBS; 6, prestained protein marker. The arrowheads point to intact FXII, HCFXII and LCFXII, respectively. (c) Binding of PK to the surface of Bacteroides. Lanes: 1, native PK; 2, B. thetaiotaomicron+PK; 3, B. thetaiotaomicron+PBS; 4, B. fragilis+PK; 5, B. fragilis+PBS; 6, prestained protein marker.