Table 2. List of primers and PCR conditions used for quantification of faecal bacteria using qPCR.
| Target group* | Sequence (5′→3′) | Size (bp) | Annealing temp (°C) | Mg2+ (millimoles l−1) | Reference |
| Universal bacteria† | F: ACTCCTACGGGAGGCAG | 468 | 60 | 2 | Zwielehner et al. (2009) |
| R: GACTACCAGGGTATCTAATCC | |||||
| Bacteroides–Prevotella–Porphyromonas‡ | See Table 1 | 140 | 68 | 2 | Rinttilä et al. (2004) |
| Bifidobacteria§ | See Table 1 | 125 | 60 | 2 | Zwielehner et al. (2009) |
| Clostridium coccoides|| | F: CGGTACCTGACTAAGAAGC | 429 | 55 | 2 | Rinttilä et al. (2004) |
| R: AGTTTYATTCTTGCGAACG | |||||
| Lactobacilli¶ | F: AGCAGTAGGGAATCTTCCA | 341 | 58 | 2 | Walter et al. (2001) |
| R: CACCGCTACACATGGAG |
F, Forward; R, reverse.
*
The following type strains were used as positive controls: †, Enterococcus faecalis (KACC 13807); ‡, Bacteroides vulgatus (KCTC 2639); §, Bifidobacterium longum; (KACC 20597); ||, R. productus (KCTC 3695); ¶, L. acidophilus (KACC 12419).